Tau-441
Tau is a family of major neuronal microtubule associated proteins that are found in the neurofibrillary tangles (NFT) in Alzheimer’s disease. Tau promotes the assembly and maintains the structure of microtubules in neuronal cells. The Tau proteins are derived from alternative mRNA splice variants that originate from a single gene and result in mature proteins that vary in size from 352 to 441 amino acids (36.8 to 45.9 kDa). There are six Tau isoforms, that differ from one another in having three or four microtubule binding repeats (R) of 31-32 amino acids each, and two, one or none amino terminal inserts (N) of 29 amino acids each. While the fetal brain contains a single isoform of tau (Tau-352) the adult brain has several isoforms all derived from a single gene by alternative mRNA splicing.
$225.00
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Product Details
- Size: 50 µg
- Physical State: White lyophilized powder
- Temperature Storage: -20°C
- Temperature Shipping: Ambient
- Sequence:MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TPPKSPSSAK SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHV PGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMV DSPQLATLAD EVSASLAKQG L
- Source: Recombinant. A DNA sequence encoding the human tau-441 (2N4R isoform) sequence was expressed in E. coli. No his-tag.
- Purity: >90% by SDS-PAGE
- Molecular Mass: 45,900 Da theoretical
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References
1. Avila, J., et al., (2004) Physiol Rev., 84 : 361
2. Goedert, M., (1993) Trends Neurosci., 16 : 460
3. Mandelkow, E., et al., (1996) Ann N Y Acad Sci., 777 : 96
4. Goedert, M., et al., (1989) Neuron., 3 : 519
5. Himmler, et al., (1989) Mol Cell Biol., 9 : 1381
6. Tai, C., et al., (2020) Neuron., 106(3) : 421-437.e11
7. Nobuhara, C., et al., (2017) Am J Pathol., 187(6) : 1399–1412 -
Publications
Highly sensitive quantification of Alzheimer’s disease biomarkers by aptamer-assisted amplification
Theranostics.; DOI: 10.7150/thno.29232.
Journal of Alzheimer’s Disease; DOI: 10.3233/JAD-2010-1271.
Mol Cell Biol.; 2010 Oct; 30 (19) :4626-43. PubMed PMID:20679486; PubMed Central PMCID: PMC2950521.
Passive Immunization with Anti-Tau Antibodies in Two Transgenic Models.
The Journal of Biological Chemistry; 2011 Sept 30 The Journal of Biological Chemistry, 286, 34457-34467.2011 Aug 12, doi: 10.1074/jbc.M111.229633.
J. Pathol.; 226: 132–142. 2012 Jan; doi: 10.1002/path.2984.
J Pharm Bioallied Sci.; 2012 Apr-Jun; 4(2): 112–117. doi: 10.4103/0975-7406.9481; PMCID: PMC3341714.
Quantification of Tau Protein Lysine Methylation in Aging and Alzheimer’s Disease.
J Alzheimers Dis.; doi: 10.3233/JAD-190604.
Highly sensitive quantification of Alzheimer’s disease biomarkers by aptamer-assisted amplification.
Theranostics; DOI: 10.7150/thno.29232.
Science Advances; DOI: 10.1126/sciadv.aau3333.
Calpain-mediated tau fragmentation is altered in Alzheimer’s disease progression.
Scientific Reports; DOI:10.1038/s41598-018-35130-y.
Prion; https://doi.org/10.1080/19336896.2018.1545524.
Tau Secretion and Propagation Is Regulated by p300/CBP via Autophagy-Lysosomal Pathway in Tauopathy.
bioRxiv; doi: http://dx.doi.org/10.1101/418640.
Anti-Tau Antibodies Based Electrochemical Sensor for Detection of Tau Protein Biomarkers.
Journal of The Electrochemical Society; DOI: 10.1149/2.0041812jes.
Translational Psychiatry; doi: 10.1038/tp.2017.165.
Neurogranin and tau in cerebrospinal fluid and plasma of patients with acute ischemic stroke.
BMC Neurol.; doi: 10.1186/s12883-017-0945-8.
BioMed Central; 10.1186/s40478-017-0435-7.
Widespread tau seeding activity at early Braak stages.
Acta Neuropathologica; doi: 10.1007/s00401-016-1644-z.
Front Neurosci.; DOI: 10.3389/fnins.2016.00504.
Naturally Occurring Autoantibodies against Tau Protein Are Reduced in Parkinson’s Disease Dementia.
PLOS ONE; http://dx.doi.org/10.1371/journal.pone.0164953.
Neurogranin and tau in cerebrospinal fluid and plasma of patients with acute ischemic stroke.
BMC Neurology; doi: 10.1186/s12883-017-0945-8.
On-chip microtubule gliding assay for parallel measurement of tau protein species.
Lab Chip; doi: 10.1039/c5lc01486g.
Rifampicin is a candidate preventive medicine against amyloid β and tau oligomers.
Oxford University Press; DOI: http://dx.doi.org/10.1093/brain/aww042.
Charles University; DOI 10.1002/jssc.201501045.
Depletion of microglia and inhibition of exosome synthesis halt tau propagation.
Nature; doi:10.1038/nn.4132.
ScienceDirect; doi:10.1016/j.exger.2015.04.010.
PLOS One; DOI: 10.1371/journal.pone.0125614.
Clinical Chemistry; doi: 10.1373/clinchem.2013.216515.
Characterization of Novel CSF Tau and ptau Biomarkers for Alzheimer’s Disease.
PLoS One; DOI: 10.1371/journal.pone.0076523.
Sensitive quantitative assays for tau and phospho-tau in transgenic mouse models.
Elsevier Inc.; doi:10.1016/j.neurobiolaging.2012.05.010.
Sensitive quantitative assays for tau and phospho-tau in transgenic mouse models.
Neurobiology of Aging; Volume 33, Issue 8, Pages 1493-1856 (August 2012) DOI 10.1016/j.neurobiolaging.2012.05.010.