Alpha-Synuclein, A53T Mutant
Alpha-Synuclein (α-Synuclein) is a 14 kD (140 amino acids) acidic presynaptic protein and is a major component of Parkinson’s Disease (PD) aggregates. α-Synuclein is implicated in the pathogenesis of PD and related neurodegenerative disorders. α-Synuclein also accumulates in the brains of sporadic PD patients as a major component of Lewy bodies, which are intraneuronal cytoplasmic inclusions characteristic of PD. α-Synuclein appears to associate with other proteins that aggregate and is found in beta-amyloid plaques and neuritic tangles in Alzheimer’s disease 3. rPeptide offers a wide array of synuclein products, including fragments, mutants, preformed fibrils, and labeled versions in addition to the wild-type protein. A point mutant (A53T) of alpha-synuclein gene that has been linked to autosomal dominant early onset Parkinson’s Disease (PD).
$300.00
-
Product Details
- Size: 0.5 mg
- Physical State: White lyophilized powder
- Temperature Storage: -20°C
- Temperature Shipping: Ambient
- Sequence:MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
- Source: Recombinant. A DNA sequence encoding the human alpha-synuclein, A53T mutant sequence was expressed in E. coli.
- Purity: >95% by SDS-PAGE and Mass Spec.
- Molecular Mass: 14,490 Da theoretical
-
References
1. Conway, K., et al., (2000) Biochemistry, 39 : 2552
2. Jakes, R., et al., (1994) FEBS Letters, 345 : 27
3. Masliah, E., et al., (2001) Proc. Natl. Acad. Sci., USA, 98 : 12245
4. Ueda, K., et al., (1993) Proc. Natl. Acad. Sci., USA, 90 : 11282
5. Grozdanov, V., et al., (2019) Ann Neurol, 86(4) : 593-606 -
Publications
NLRP3 Inflammasome Is Expressed and Functional in Mouse Brain Microglia but Not in Astrocytes.
PLOS ONE; DOI: 10.1371/journal.pone.0130624.
Prion; doi: 10.1080/19336896.2020.1724608.
Mol Neurobiol.; DOI: 10.1007/s12035-019-1567-5.
Protein Damage in Aging and Age-Related Diseases.
Open Biology; https://doi.org/10.1098/rsob.180249.
Springer Berlin Heidelberg; https://doi.org/10.1007/s00401-019-01993-2.
α‐Synuclein activates innate immunity but suppresses interferon‐γ expression in murine astrocytes..
Eur J Neurosci.; https://doi.org/10.1111/ejn.13956.
DOPAL is transmissible to and oligomerizes alpha-synuclein in human glial cells..
NCBI; doi: 10.1016/j.autneu.2015.12.008.
Mapping of Surface-Exposed Epitopes of In Vitro and In Vivo Aggregated Species of Alpha-Synuclein.
Springer Link Cellular and Molecular Neurobiology; doi:10.1007/s10571-016-0454-0.
JBC Papers in Press; doi:10.1074/jbc.M115.641746.
Role of Matrix Metalloproteinase 3-mediated α-Synuclein Cleavage in Dopaminergic Cell Death.
J Biol Chem.; 2011 Apr 22;286(16):14168-77. Epub 2011 Feb 17..