FITC Beta-Amyloid (1-42) Preformed Fibrils

Product Background: Beta-Amyloid peptides (A-beta), the major constituent of amyloid plaques in the brains of Alzheimer’s patients, are thought to be a primary contributor of Alzheimer’s Disease (AD).

About the product: While some fluorescent Beta-Amyloid on the market uses intrinsic fluorescence and adds in tryptophan residues by mutations, rPeptide’s human Beta-Amyloid has been covalently labeled with Fluorescein Isothiocyanate and assayed for fluorescence. Lysine residues of the native Beta-Amyloid sequence were used as labeling sites, leaving the cysteine residues unaffected. This FITC labeled form of Beta-Amyloid (1-42) was then formed into fibrils according to rPeptide’s proprietary methods. Fibril formation is determined by TEM and thioflavin assay, while fluorescence is confirmed before fibrillization by end point fluorescence assay and after by LM.

Applications: These FITC Beta-Amyloid (1-42) Preformed Fibrils are ideal for experimental use or as controls in aggregation kinetics, fibril morphology, seeding experiments, or in vivo imaging.