FITC Alpha-Synuclein Preformed Fibrils
Product Background: Alpha-Synuclein (α-Synuclein) is a presynaptic protein found to be a major component of Parkinson’s Disease (PD) aggregates and is implicated in the pathogenesis of PD and related neurodegenerative disorders.
About the product: While some fluorescent Alpha-Synuclein on the market uses intrinsic fluorescence or adds in tryptophan residues by mutations, rPeptide’s human Alpha-Synuclein has been covalently labeled with Fluorescein Isothiocyanate and assayed for fluorescence. Lysine residues of the native Alpha-Synuclein sequence were used as labeling sites, leaving the cysteine residues unaffected. This FITC labeled form of human Alpha-Synuclein was then formed into fibrils according to rPeptide’s proprietary methods. Fibril formation is determined by TEM and thioflavin assay.
Applications: The FITC Alpha-Synuclein fibrils are sold in 200mM Tris, 250mM NaCl, pH 7.4 and may be suitable for experimental use or as controls in aggregation kinetics, fibril morphology, seeding experiments, or in vivo imaging.
Price range: $635.00 through $6,900.00
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Product Details
- Physical State: Liquid
- Temperature Storage: -80°C
- Temperature Shipping: Dry Ice
- Sequence:
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
- Source: Recombinant. A DNA sequence encoding the human Alpha-Synuclein sequence was expressed in E. coli with FITC molecules attached for fluorescence. This fluorescent monomer underwent proprietary fibrillization procedures.
- Purity: >95% by SDS-PAGE
- Molecular Mass: N/A. See Estimated Size on Data Sheet
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References
- Roberi, M., et al., (2007), Nature Methods, 4, 345-351
- Chung, C., et al., (2022), Analytical Chemistry, 94 13
- 3. Reynolds, N., et al., (2011), Journal of the American Chemical Society, 133, 48