Biotin Tau-441

Tau is a family of major neuronal microtubule associated proteins that are found in the neurofibrillary tangles (NFT) in Alzheimer’s disease. Tau promotes the assembly and maintains the structure of microtubules in neuronal cells1,2,3. An avi-tag was added to the C-terminus of the tau-441 sequence for in vivo biotinylation of the protein. Biotinylation was verified by ELISA and western blot. Biotinylated tau-441 can serve as a probe in many research applications including immunohistochemistry, localization studies4, and affinity assays5, without the need for further labeling or a specific antibody.

Catalog ID: T-1114-2

$800.00

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  • Product Details
    • Size: 100 µg
    • Physical State: White lyophilized powder
    • Temperature Storage: -20°C
    • Temperature Shipping: Ambient
    • Source: Recombinant. A DNA sequence encoding the human tau-441 (2N4R isoform) sequence was expressed in E. coli and biotinylated on the C-terminus via BirA.
    • Purity: >90% by SDS-PAGE.
    • Molecular Mass: 47,904 Da

  • References

    1.Avila, J., et al., (2004) Physiol Rev., 84 : 361 2.Goedert, M., (1993) Trends Neurosci., 16 : 460 3.Mandelkow, E., et al., (1996) Ann N Y Acad Sci., 777 : 964.Shivastava, A., et al., (2019) EMBO J, 38(3) : e998715.Roberts, M., et al., (2020) Acta Neuropathol Commun, 8 : 13

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