Biotin Tau-352
Tau is a family of major neuronal microtubule associated proteins that are found in the neurofibrillary tangles (NFT) in Alzheimer’s disease. Tau promotes the assembly and maintains the structure of microtubules in neuronal cells1,2,3. An avi-tag was added to the C-terminus of the tau-352 sequence for in vivo biotinylation of the protein. Biotinylation was verified by ELISA and western blot. Biotinylated tau-352 can serve as a probe in many research applications including immunohistochemistry, localization studies4, and affinity assays5, without the need for further labeling or a specific antibody.
$550.00
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Product Details
- Size: 50 µg
- Physical State: White lyophilized powder
- Temperature Storage: -20°C
- Temperature Shipping: Ambient
- Sequence:MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKAEEAGI GDTPSLEDEA AGHVTQARMV SKSKDGTGSD DKKAKGADGK TKIATPRGAA PPGQKGQANA TRIPAKTPPA PKTPPSSGEP PKSGDRSGYS SPGSPGTPGS RSRTPSLPTP PTREPKKVAV VRTPPKSPSS AKSRLQTAPV PMPDLKNVKS KIGSTENLKH QPGGGKVQIV YKPVDLSKVT SKCGSLGNIH HKPGGGQVEV KSEKLDFKDR VQSKIGSLDN ITHVPGGGNK KIETHKLTFR ENAKAKTDHG AEIVYKSPVV SGDTSPRHLS NVSSTGSIDM VDSPQLATLA DEVSASLAKQ GL
- Source: Recombinant. DNA sequence encoding the human Tau-352 isoform (0N3R) sequence was expressed in E. coli and biotinylated on the C-terminus via BirA.
- Purity: >90% by SDS-PAGE.
- Molecular Mass: 36,800 Da for native Tau 352
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References
1.Avila, J., et al., (2004) Physiol Rev., 84 : 361
2.Goedert, M., (1993) Trends Neurosci., 16 : 460
3.Mandelkow, E., et al., (1996) Ann N Y Acad Sci., 777 : 96
4.Shivastava, A., et al., (2019) EMBO J, 38(3) : e99871
5.Roberts, M., et al., (2020) Acta Neuropathol Commun, 8 : 13
