C13, N15 Tau-441 (2N4R) Uniformly Labeled

T-1103-2

–20C

Recombinant. DNA sequence encoding the human Tau-441 isoform (2N4R) sequence was expressed in E. coli with N15 as the nitrogen source and C13 as the carbon source. No his-tag.

Tau is a family of six isoforms, derived from a single gene by alternative mRNA splicing ¹. They vary in size from 352 to 441 amino acids (36.8 to 45.9 kDa) and differ from one another in having three or four microtubule binding repeats (R) of 31-32 amino acids each, and two, one or none amino terminal inserts (N) of 29 amino acids each². Tau promotes the assembly and maintains the structure of microtubules in neuronal cells^3,4,5. While the fetal brain contains a single isoform of tau (Tau-352) the adult brain has several isoforms. Tau is both phosphorylated and O-GlcNAcylated6. The normal brain tau contains 2-3 moles of phosphate/mole of the protein. In Alzheimer disease tau is hyperphosphorylated, containing 3-4-fold more phosphate/mole of the protein than the normal tau^7,8 and is the major protein subunit of paired helical filaments (PHF) that form the neurofibrillary tangles (NFT). NFT accumulation correlates with the clinical progression of Alzheimer's disease.

48.4 kDa

>90% by SDS-PAGE.

$2,699

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