Product Name
13C, 15N Tau-441 (2N4R) Uniformly Labeled
Size
Catalog #
T-1103-2
Storage
–20C
Sequence
MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TPPKSPSSAK SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHV PGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMV DSPQLATLAD EVSASLAKQGL
Source
Recombinant. DNA sequence encoding the human Tau-441 isoform (2N4R) sequence was expressed in E. coli with N15 as the nitrogen source and C13 as the carbon source. No his-tag.
Description
Tau is a family of six isoforms, derived from a single gene by alternative mRNA splicing1. They vary in size from 352 to 441 amino acids (36.8 to 45.9 kDa) and differ from one another in having three or four microtubule binding repeats (R) of 31-32 amino acids each, and two, one or none amino terminal inserts (N) of 29 amino acids each2. Tau promotes the assembly and maintains the structure of microtubules in neuronal cells3,4,5. While the fetal brain contains a single isoform of tau (Tau-352) the adult brain has several isoforms. Tau is both phosphorylated and O-GlcNAcylated6. The normal brain tau contains 2-3 moles of phosphate/mole of the protein. In Alzheimer disease tau is hyperphosphorylated, containing 3-4-fold more phosphate/mole of the protein than the normal tau7,8 and is the major protein subunit of paired helical filaments (PHF) that form the neurofibrillary tangles (NFT). NFT accumulation correlates with the clinical progression of Alzheimer's disease.
Molecular Mass
48.4 kDa
Purity
>90% by SDS-PAGE.
Cost (US$)
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1. Himmler, et. al., 1989, Mol Cell Biol. 9, 1381
2. Goedert, M., et. al. 1989, Neuron. 3, 519.
3. Avila J. et. al., 2004, Physiol Rev. 84, 361.
4. Goedert, M., 1993, Trends Neurosci. 16, 460.
5. Mandelkow, E. et al. 1996, Ann N Y Acad Sci. 777, 96.
6. Liu, F., et. al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101, 10804.
7. Iqbal, K., et. al., 1986, Lancet 2, 421.
8. Kopke, et. al., 1993, J. Biol. Chem. 268, 2437
2. Goedert, M., et. al. 1989, Neuron. 3, 519.
3. Avila J. et. al., 2004, Physiol Rev. 84, 361.
4. Goedert, M., 1993, Trends Neurosci. 16, 460.
5. Mandelkow, E. et al. 1996, Ann N Y Acad Sci. 777, 96.
6. Liu, F., et. al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101, 10804.
7. Iqbal, K., et. al., 1986, Lancet 2, 421.
8. Kopke, et. al., 1993, J. Biol. Chem. 268, 2437