N15, Alpha-Synuclein, Uniform Label
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Recombinant. A DNA sequence encoding the human alpha-synuclein sequence was expressed in E. coli, with N15 as the nitrogen source.
15N Uniform labeled Alpha-Synuclein (α-Synuclein) has all nitrogen’s
N15 labeled. α-Synuclein is a 14 kD (140 amino acids) acidic presynaptic
protein. It is a major component of Parkinson’s disease aggregates and is
implicated in the pathogenesis of Parkinson’s Disease and related
neurodegenerative disorders. α-Synuclein accumulates in the brains of
sporadic Parkinson’s disease patients as a major component of Lewy
bodies, which are intraneuronal cytoplasmic inclusions characteristic of
Parkinson’s disease. α-Synuclein appears to associate with other proteins
that aggregate and is found in β-amyloid plaques and neuritic tangles in
>95% by SDS-PAGE and Mass Spec.