Do you run mass specs for Taus or only gel?
Purity assay for tau is done by SDS only, no mass spec.
Do your Tau Proteins have his tags?
Our Tau proteins do not have his tags.
Advantage of Tau proteins without his tag vs tau proteins with his tag:
1. Tau protein with his-tag is more likely to be insoluble and hence inamenable for various biochemical assays. This problem will not be there in the Tau protein without any tag.
2. Tau protein with his-tag is more likely to have contaminating proteins which will be coming due to the non-specific nature of interaction between polyhistidine tags and Ni2+ ions. These proteins will interfere with any of the assays or functions of Tau proteins.
3. There are chances that some Nickel too can come as contaminant which can severely alter the functions of Tau.
4. A his-tag Tau can also be immunogenic if used in humans. There can be immune response against the polyhistidine tag which has the potential to act as a linear epitope.
How can your Taus be used?
Our products are not for human use, they are for research purposes only.
How should your lyophilized Tau be stored?
The lyophilized Tau can be stored for at least 2 years at -80°C.
How should your Tau be stored after reconstitution in water?
After reconstitution in water, its best to aliquot it (AVOID FREEZE THAW) and store at -80°C. This should be stable for about 1-2 weeks. For longer term, its best to lyophilize it.
What is the stability of lyophilized Taus?
The lyophilized Tau is stable for at least 2 years at -80°C. After reconstitution in water, its best to aliquot it (AVOID FREEZE THAW) and store at -80°C. This should be stable for about 1-2 weeks. For longer term, its best to lyophilize it.
Which one of the Tau protein isoforms that you sale is the longest or which ones are the most phosphorylated?
Tau 441 is the biggest tau that we have. Since they are made in E.coli they are not phosphorylated.